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In addition, for negative controls, binding of ECFP alone with EYFP alone (or ECFP alone with EGFP alone and DsRed monomer alone) was analyzed.
This study also demonstrated that proteolytic processing controls binding of Sema3C to its receptor.
As controls, binding of the methyl esters of alanine and aspartate residues was also examined under selected conditions.
At the same time it became sensitive to the rate of transcription activation (k sr,i ) induced by the receptor-tamoxifen complexes (ER2I, ER2I2), and acquired negative sensitivity to the parameter K18, which controls binding of ER2I complexes to ERE.
The regulatory Ser phosphorylation site on NR controls binding of a 14-3-3 14-3-3 14-3-3orm a catalytically inactive comproteinR-pSer–14-3-3) toat modulates NR activity in response to light and other environmental signals [ 45, 46].
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An important prediction of the accessibility model is that cis-elements and transcription control binding of the recombination-activating gene 1 (Rand) and RAG2 proteins to their DNA targets.
As a control, binding of PAI-1 to SMTB 1 44 was measured by ITC with the results shown in Figure 4, A and B. Fitting of the observed enthalpies to a single-site binding model revealed a KD of <1.0 nM for PAI-1 binding to SMTB 1 44.
Interestingly, CpG methylation status appeared to control binding of MYB to the WNT5A promoter region.
As a negative control, binding of the factors was also monitored at an irrelevant DNA region (negative control [NC] DNA).
It follows that understanding the factors that control binding of apoA-I to HDL particles is significant.
To demonstrate spatially controlled binding of targeted particles, matrices were evaluated by imaging sections prepared in OCT.
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