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To discriminate changes in modules comparing SZP and CTS, we performed a module Preservation analysis that identified which modules were preserved in both datasets.
Second, we also replicated module preservation analysis over individual GEO datasets by assuming that they were test datasets.
Our module preservation analysis is based on the modulePreservation R function implemented in the WGCNA R package.
Our module preservation analysis is based on a permutation test implemented in the modulePreservation R function [ 30].
Module preservation analysis was performed to investigate if density and connectivity based network measures were preserved across data sets and conditions [ 40].
Modules are labeled if they were preserved in independent in vivo and in vitro expression data sets [ 41, 42] according to module preservation analysis [ 40] (Methods).
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A functional enrichment analysis of brain module preservation reveals basic functional pathways preserved between the two tissues.
This systems biologic study also illustrates how comparative WGCNA analysis (based on module preservation statistics) can be used to assess the suitability of a rodent model with respect to human disease-related transcriptional changes.
Thus, the higher the value of the module preservation measure, the better preserved is the module structure (its connectivity pattern) between the two strains.
Here we pursue a more comprehensive analysis that not only quantifies module preservation but also determines intermodular preservation.
Analysis of statistics of network module preservation determines whether modules that were identified in one network (reference) remain connected in another network (test) and yields a Z-summary statistic: a composite measure of statistics related to network density and connectivity.
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module detection analysis
module strength analysis
module preservation measure
module preservation functionality
module preservation study
module preservation calculation
module preservation test
module level analysis
module validation analysis
module network analysis
module evolution analysis
module enrichment analysis
module stability analysis
module map analysis
module pathway analysis
module complexity analysis
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