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Since the module preservation calculation is computationally time-consuming, we did not perform other algorithms for comparison.
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The R package WGCNA implements a suite of tools for network construction, module detection, module significance examination, module preservation computation, and hub gene query, among many others [ 29– 31].
We used the modulePreservation function from the WGCNA library to calculate module preservation statistics [ 31].
First, the significantly changed proteins of B73 were used to calculate the "module preservation" of 18R as illustrated above.
Thus, the higher the value of the module preservation measure, the better preserved is the module structure (its connectivity pattern) between the two strains.
The modules are based on one condition BE or AE and their module preservation scores in another condition are calculated.
A functional enrichment analysis of brain module preservation reveals basic functional pathways preserved between the two tissues.
The function, module Preservation, in the WGCNA package was used to calculate the Z statistics.
To discriminate changes in modules comparing SZP and CTS, we performed a module Preservation analysis that identified which modules were preserved in both datasets.
A high degree of module preservation between the prenatal animals was observed by calculating the overlap for each possible pair of modules (Additional file 1: Table S3).
The module preservation of 18R modules in the B73 data was calculated, which can also be regarded as a differential network analysis.
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