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This study showed how strong the effect of using strategic gTME with the importance of the modification we conducted in Error Prone PCR protocol by increasing MnCl2 concentration instead of MgCl2.
To test for effect modification, we conducted a pre-planned stratified analysis based on coexisting diabetes.
Because of the potential regulatory role of the modification, we conducted this analysis in synchronous cultures to determine whether SUMOylation levels of Tpz1 vary during the cell cycle.
To evaluate effect modification, we conducted analyses separately for subgroups defined by BMI (<25, 25 30, >30) at the baseline interview and stage of breast cancer (local, regional or distant) at diagnosis.
To evaluate effect modification, we conducted analyses separately for subgroups defined by BMI (<25, 25 30 and >30) at the baseline interview and stage of breast cancer (local, regional or remote) at diagnosis.
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Because global histone acetylation changes preceded alterations in other histone modifications, we conducted ChIP assays in colon cancer cells at an early time point, 4 h.
To investigate whether there was a relationship between sample size and evidence for effect modification, we also conducted the LRT for effect modification between APOE genotype and smoking status in each study individually, and plotted the P-values derived from LRT against sample size, grouped by whether the original publication reported evidence of effect modification.
To check for effect modification by sex, we conducted fully adjusted analyses stratified by sex.
Because of possible effect modification by diabetes status, we conducted analyses overall and stratified by a diagnosis of diabetes.
After a few modifications in the setup, we conducted a whole registry in 2010.
To understand the reason for this discrepancy, we conducted global modification analysis [ 37] of the dominant isomiR sequences in our study and found out that single nucleotide 3'extensions were the most prevalent type of modifications while 5'end modifications were much lower (Additional file 7a).
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