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Thus, we developed an adapted BFP model using primary T cells and an artificial model APC coated with antibodies specific for the TCR/CD3 complexes and/or LFA-1 molecules.
An in vitro BBB model using primary cells isolated from rat brains was established.
We developed a GEM-derived orthotopic model using primary brain tumor cells that had been isolated from induced TRP mice.
Our model, using primary cultures of human corneal stroma keratocytes, also has the advantage of being fully isogenic.
A similar reduction in leukemia burden was observed with a neutralizing CXCR4 antibody in a xenograft model using primary human leukemic cells.
Because most of the data are from animal studies, we established a model using primary normal human neural progenitor (NHNP) cells to determine if PCBs interfere with TH-dependent neural differentiation.
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5, 6 Cell culture models using primary human cells are valuable screening tools for both risk assessment purposes and mechanistic investigations.
We have previously shown that BMS is the most potent chemoattractant for metastatic PECs and that the metastatic process can be modelled using primary human BMS co-cultures (Hart et al, 2005; Brown et al, 2006).
Indeed, orthotopic tumour models, using primary tissue, have been established for several cancer subtypes [ 15- 18], and studies have demonstrated that they can recapitulate tumour-host interactions and display traits of the parent tumour [ 19].
Cell-based in vitro BBB models using primary or immortalized brain capillary endothelial cells from animal or human origin cultivated on microporous filter membranes of Transwell systems may bridge the gap between in silico and in vivo studies.
In vitro animal BBB models using primary or low passage porcine, bovine, rat, or mouse cells, partly in double and triple co-culture systems, are generally characterized by relatively high TEER values and by low paracellular permeability of marker compounds [ 17].
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