Exact(4)
5, 6 Cell culture models using primary human cells are valuable screening tools for both risk assessment purposes and mechanistic investigations.
Indeed, orthotopic tumour models, using primary tissue, have been established for several cancer subtypes [ 15- 18], and studies have demonstrated that they can recapitulate tumour-host interactions and display traits of the parent tumour [ 19].
Cell-based in vitro BBB models using primary or immortalized brain capillary endothelial cells from animal or human origin cultivated on microporous filter membranes of Transwell systems may bridge the gap between in silico and in vivo studies.
In vitro animal BBB models using primary or low passage porcine, bovine, rat, or mouse cells, partly in double and triple co-culture systems, are generally characterized by relatively high TEER values and by low paracellular permeability of marker compounds [ 17].
Similar(4)
Some reported in vitro models use primary cells, while others use immortalized cell lines.
The models use primary porcine choroid plexus epithelial cells (PCPEC) and human choroid plexus papilloma cells (HIBCPP).
Thus, we developed an adapted BFP model using primary T cells and an artificial model APC coated with antibodies specific for the TCR/CD3 complexes and/or LFA-1 molecules.
An in vitro BBB model using primary cells isolated from rat brains was established.
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