This hypothesis could be appropriately tested by using primary specimens containing both tumor and adjacent normal tissues, not by cultured cells.
The cellular localisation of the expression of the deregulated genes was evaluated using primary malignant epithelial and stromal cell cultures derived from radical prostatectomy specimens.
These signature genes for melanoma progression are remarkably similar to those obtained from recent large-scale studies using primary human melanomas and suggest high correlation with alterations seen in primary tumor specimens [10].
using primary bovine endometrial cells.
Staining was performed on formalin-fixed, paraffin-embedded specimens using anti-ERBB2 primary antibody (DAKO, Carpinteria, CA, USA).
In this communication, we analyzed solid tumor cancer stem cell markers in human breast cancer cell lines and primary specimens using flow cytometry.
Data for MPRED were available from only one study (Styczynski et al, 2002), Whilst MTX sensitivity cannot be measured in primary specimens using the MTT assay (Pieters et al, 1997).
Methotrexate sensitivities have been successfully compared in primary specimens using in situ inhibition of thymidylate synthetase (Rots et al, 2000), but the data are not comparable to the growth inhibition studies performed in the present study.
Flow cytometric cell testing and sorting was performed on single-cell suspensions derived from cell lines and primary tumor specimens using an Epics Altra flow cytometer (Beckman Coulter).
RNA was extracted from infected tissue culture supernatants using Tri-Reagent (Molecular Research Center, Inc., Cincinnati, OH, USA) and from primary clinical specimens using the High Pure Viral Nucleic Acid kit (Roche Diagnostics GmbH, Mannheim, Germany).
