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We set out to study the mobility of endogenous Eag1 channels in the plasma membrane of hippocampal neurons using SPT.
In the last years, technical advances using quantum qots (QD) nanocrystals have made it possible to study the mobility of endogenous ion channels in neurons at high temporal and spatial resolution.
Finally, the comparison between the mobility of endogenous AGS3 and that of a recombinant AGS3 (Figure 3D) further shows that the slowest-migrating band of the triplet is most likely covalently modified.
The mobility of endogenous NFAT5 in HEK293 was faster than that of the recombinant HA-NFAT5a expressed in the same cell line and clearly faster than that of HA-NFAT5c (Figure 7A).
According to this paradigm, the primary role of cytosine methylation was evolved to serve as a genome defense system particularly to control mobility of endogenous TEs, and therefore stress-induced alteration in scope and/or extent of this epigenetic marker may provoke activation of otherwise quiescent TEs [ 43]. * and ** respectively indicate statistical significance at the 0.05 and 0.01 levels.
A very recent paper [ 15] has also shown that okadaic acid reduced the mobility of endogenous MST3 in HEK (human embryonic kidney -293 cells [detected using mAbs to an epitope in the C-terminus of MST3 FL)], which was reversed by incubation with exogenous purified PP2A.
Similar(54)
To directly test whether the change in Fra-2 mobility with CT-1 was due to direct phosphorylation, we analyzed mobility changes of endogenous and exogenously expressed Fra-2 in the presence or absence of CT-1 in C2C12 MB cells.
Because Ca2+ is well buffered with an estimated binding ratio of 65, the mobility of the endogenous mobile buffers must be high to explain the high apparent mobility of Ca2+.
Expression of dominant-negative Atg1 in fat bodies of fed larvae reduced the amount of slower mobility forms of endogenous Atg13, and in starved larvae practically eliminated all Atg13high forms (Fig. 7E; Fig. S4G).
188 Similarly, incorporation of T. brucei variant surface glycoprotein (VSG) into baby hamster kidney cells showed that the inserted VSG exhibited lateral mobility equivalent to that of endogenous VSG in T. brucei.
Instead, they possibly reflect the endogenous mobility of the CTDs in the Kir6.2-inhibited state, because we observed similar conformational heterogeneity of the KATP channel in the ATP state and the Mg-ADP state as described later.
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