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Exact(6)
Working solution (0.2 mg/mL) [for HPLC DAD] was then prepared in the mobile phase mixture of acetonitrile water (40 60, v/v).
Using a binary mobile phase mixture (methanol water, 70 30, v/v) the velocity of the mobile phase front on the HPTLC RP-18W plates at different temperatures was investigated.
After vortexing, the mobile phase mixture was transferred to polypropylene microliter inserts.
The API 4000 instrument was operated with a flow rate of 0.35 mL/min, using a mobile phase mixture similar to that used in the analysis of PFOA as described above.
Samples were processed by organic phase extraction in hexane and retinol was isolated from the extract using a C18 column (150 mm × 4.6 mm, 3 μm particle size) with a mobile phase mixture of acetonitrile, tetrahydrofuran, methanol and 1% ammonium acetate solution (68 22 7 3) flowing at 1 ml/min.
Briefly, we used a Perkin-Elmer LC200 quaternary system run isocratically using a reverse-phase column (Synergi 4 μ Fusion-RP80A, 250 × 4.6 mm) plus guard column from Phenomenex Ltd., a mobile phase mixture of methanol and sodium dihydrogen phosphate buffer (27:73, by volume), final pH 2.8, at a flow rate of 1.2 ml/min at 37°C, and a uv/vis detector (220 nm).
Similar(54)
Briefly, the crude ethanolic extract was subjected to silica column chromatography, using varying polarity of mobile phase mixtures and collection of fractions.
The design was applied to the simultaneous optimization of both mobile phase chromatographic mixtures and extraction mixtures for the Camellia sinensis Chinese tea plant.
The design is applied to the simultaneous optimization of both mobile phase chromatographic mixtures and extraction mixtures for the Baccharis milleflora (Less).
As mobile phase a mixture of 100% methanol (mobile phase A) and water supplemented with 0.1% formic acid (v/v) (mobile phase B) was used.
Chromatographic separation was achieved on Kromasil® 100-5C18 using a mobile phase consisting mixture of pH 6.0 phosphate buffer and acetonitrile (60 40 v/v) under isocratic mode of elution.
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