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The detector temperature directly affected the volatilization of the carrier mobile phase solvent.
The aqueous mobile phase, solvent 'A', was prepared as a 0.1% (v/v) formic acid (FA) in dH2O, and the organic mobile phase, solvent 'B', was prepared as 0.1% (v/v) FA in acetonitrile.
The aqueous mobile phase, solvent 'A', was prepared as a 0.1% (v/v) FA in dH2O, and the organic mobile phase, solvent 'B', was prepared as 0.1% (v/v) FA in acetonitrile.
The mobile phase, solvent A (ultrapure water) and solvent B (acetonitrile), was delivered at a flow rate of 1.0 mL min-1.
The mobile phase solvent used was 5 mM sulphuric acid, the temperature was maintained at 60 °C and the flow rate was 1.0 mL/min.
Individual standard solutions (10 mg L−1) were infused with the syringe pump and mixed using a tee with the LC flow, mobile phase solvent A: 0.1 % NH4OH, H2O and solvent B: 0.1 % NH4OH, MeOH (50 50), (300 μL min−1), before being introduced into the HESI source.
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The method uses a pump to force one or more mobile phase solvents through high-efficiency, tightly packed columns.
Use of different mobile phase solvents was investigated.
Water and acetonitrile both containing 0.1% formic acid were used as mobile phase solvents.
Mobile phase solvents comprised of water with 0.001% v/v formic acid as solvent A and 50 50 mixture of acetonitrile and methanol as solvent B. The flow rate of mobile phase through the column was 300 μL/min.
Mobile phase solvents were filtered through a 0.2 μm nylon filter.
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