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Mobile phase solution A consisted of 0.1% ortho-phosphoric acid in water and Solution B consisted of 100% acetonitrile.
Mobile phase solution was methanol/0.1 M NaH2PO4 (2:1), pH 3.2 (according to Shephard et al. 1991).
Mobile phase solution was methanol/0.1 M NaH2PO4 pH 3.2 (2:1) at a flow rate of 0.7 ml/min (according to [Shephard et al. 1991]).
The dried extracts were reconstituted with 0.50 mL of the mobile phase solution of ammonium bicarbonate (5 mM) pH 7.8 – acetonitrile (1 1 v/v) in injection vials and later injected in LC-QqQMS or LC-QqToFMS for analysis.
The final extracts were reconstituted in 0.5 mL of the mobile phase solution of ammonium bicarbonate (5 mM) pH 7.8 – acetonitrile (1 1 v/v), filtered with a PTFE 0.45 μm filter, and then injected in LC-QqQMS system for analysis.
The dried extract was resolubilized in 1 3 mL of mobile phase solution and filtered (0.22 μm PTFE membrane).
Similar(51)
The mobile phase solutions were H2O and ACN, both containing 0.1% (v/v) formic acid, and delivered at a constant flow rate of 0.2 µl/min.
The mobile phase contained solution A of 0.5 % formic acid and solution B of acetonitrile, with a column temperature of 35 °C and flow rate of 0.3 mL/min.
Mobile phase: a solution consists of 0.15 M SDS, 8% n-propanol, 0.3% TEA, prepared in 0.02 M orthophosphoric acid.
The separation was performed on an anion exchange column PRP-X100 using a gradient elution program between EDTA/KHP (potasium hydrogen phtalate) as first mobile phase and phosphate solutions solution as the second one.
The mobile phase consisted of solution A (0.1 M ammonium formate (pH 6.7)) and solution B (1 1 acetonitrile/methanol) and a gradient from 0% B to 80% B in 40 minutes at the flow rate was 1 mL/min was used to obtain the chromatographs.
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