We took fermentation samples at twenty-four-hour intervals beginning from 0 h, and analyzed them using HPLC (Waters Corporation, Milford, MA) with a Shodex RSpak KC-811 column and 0.1%H3PO44 as the mobile phase (1.0 mL/min, 60°C).
The molecular weight distributions of the acetylated lignin samples were then analyzed on an Agilent GPC SECurity 1200 system equipped with four Waters Styragel columns (HR1, HR2, HR4, HR6), an Agilent refractive index (RI) detector, and an Agilent UV detector (270 nm), using tetrahydrofuran (THF) as the mobile phase (1.0 mL/min), with an injection volume of 20 μL.
Table 5 Experimental conditions for HPLC analysis of PXD in soil and wheat grain samples Experimental conditions Method Solvent Injection volume (μL) Mobile phase Flowrate (mL/min) Wavelength (nm) t r (min) Method 1 Siebers et al. 2014 CH3CN 10CH3CN/0.1%H3PO450+503.050 3.0 260 2.6 Method 2 Shehzad and Shah 2013 CH3CN 20 CH3CN/CH3OH 50 + 50 500 260 6.0.
The compound was purified by high-pressure liquid chromatography (HPLC) under the following conditions: column TSK-GEL amide-80, 5 μm (Waters, Milford, MA, USA); 80% (v/v) acetonitrile in water mobile phase; 1.0 mL/min flow rate; RID-10A RI detector (Shimadzu, Tokyo, Japan).
The mobile phase (1 mL/min) was 22 mmol/L NaOH.
Sulfuric acid (5 mM) was used as the mobile phase (0.6 ml/min), and the oven temperature was 40°C.
The samples were injected with a PL-AS RT Autosampler for PLGPC 50 Plus using chloroform as the mobile phase (1 mL/min, 30 °C).
Mobile phase (1 mL/min) was 3.65 mM phosphoric acid, internal standard was phenoxyacetic acid and peak detection was done with ultraviolet (210/280 nm).
Sugar Pak I guard and analytical columns were used at 80°C with ultrapure water as a mobile phase (0.5 mL/min).
The samples were injected using a PL-AS RT Autosampler for a PLGPC 50 Plus using chloroform as the mobile phase (1 mL/min, 30 °C).
