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Exact(28)

The prepared sample solution (20 mL) was loaded and injected directly into CCC column for final separation.

The concentrated crude extract (5 mL) was loaded onto a QAE-Sephadex A-50 column (30 × 2.5 cm) pre-equilibrated with 20 mM glycine NaOH buffer (pH 8.0).

Briefly, the column was conditioned with 5 ml of methanol and MQ-water, then the sample (5 ml) was loaded on the column.

The concentrated extract (30 ml) was loaded onto a DEAE cellulose column (18 cm × 3.0 cm) pre-equilibrated with 10 mM acetate buffer (pH 4.8).

Plasma was separated from blood cells and, between 0.3 and 0.7 mL, was loaded onto a tC-18 Sep-Pak (Waters, The Netherlands) and washed with 20 mL of water.

The supernatant (∼1 mL) was loaded onto a 0.2 M to 1.2 M linear sucrose gradient and centrifuged at 25,000 r.p.m. in a Beckman rotor (SW41) for 15 minutes (after reaching the final speed).

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Similar(32)

OLA (1 mL), Cadmium precursor (2 mL), and ODE (2 mL) were loaded into a 50-mL three-neck flask.

Lipid nanoparticle suspensions of 0.2, 0.3, 0.5, and 1 mL were loaded in column with water elution.

For the preparation of CuInS2 QDs, InCl3 · 3H2O (0.1 mol), CuCl2 · 2H2O (0.1 mol), sulfur (0.1 mol), and ODE (200 ml) were loaded into a 500-ml three-necked flask.

Se (6 mmol) and ODE (60 mL) were loaded in a 100 mL three-necked flask, and then heated to 220 °C for 180 min under nitrogen to obtain yellow clear solution.

Between 0.4 and 0.45 mL were loaded for each sample.

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