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Five milliliters was loaded at a time onto a Superdex 200 Hi Load 26/60 gel filtration column (GE Healthcare) pre-equilibrated with 10 20 mM CAPS at pH 10.5, 100 mM KCl, and 2 mM DTT.
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A disposable glass reaction cell, in which sub-milliliter titrand was loaded, was inserted into the working head stage of the C4D.
Forty milliliters of sample was loaded each time.
One milliliter of SLN dispersion was loaded in the dialysis tube, and the dialysis tube tied firmly at both ends was immersed in 50 mL phosphate-buffered saline (PBS) (pH 3.5 and 7.4).
One milliliter of the solution was loaded to a Zetasizer (Nano ZS, Malvern, Worcesteshire, UK) for the zeta potential measurement at 25 °C.
Two milliliters of each of TET solution and the TET-loaded LCNP formulation was loaded separately into the dialysis bags.
One thousand milliliters of collected sample, which was eluted from the gel-filtration column, was loaded onto a SP-Sepharose FF column (2.6 cm × 20 cm), which was also equilibrated with 20 mmol/L acetate buffer (pH 5.0).
Two milliliters of the supernatant were loaded onto a 1 g C18 sample preparation cartridge (Alltech Associates, Inc., Deerfield, IL).
Two milliliter sterile screw-cap tubes were loaded with silicon carbide sharps of sizes 0.1 mm and 1 mm (BioSpec Products, Inc., Bartlesville, OK) at a 1 1 ratio up to a volume equivalent to 250 μl.
Three milliliters of ODE and 1 g ODA are loaded into a flask followed by adding the synthesized CdSe nanocrystals (preserved in hexane).
Two milliliters of this solution (corresponding to 6 g wet sample) were loaded onto a GPC column.
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