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The top 1 mL was discarded, and the remainder was split in ~0.9 mL aliquots for the following experiments.
The initial 20 ml was discarded.
The top 3 ml was discarded.
Lavage fluid from the first 20 mL was discarded.
A blood volume of 0.5 mL was discarded before sample collection to avoid drug dilution effects.
After an equilibration period of 30 minutes, 1.0 ml was discarded.
Similar(45)
The flow through and the first 1.5 mL were discarded.
The first 20 ml were discarded to decrease potential seawater contamination during sampling.
For evaluation of EPCs, blood samples were drawn from an antecubital or existing central vein; the first 3 ml were discarded.
The first 2 mL were discarded and the remaining 18 mL were transferred in plastic tubes containing 0.105 M sodium citrate.
The lyophilised vaccine or placebo was reconstituted in either 150 ml (for children above 10 years) or in 75 ml of the bicarbonate solution (the other 75 ml were discarded) for children below 10 years and was administered immediately.
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