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Whole blood (10 ml) was collected by venepuncture into plain collection tubes for serum (blood was allowed to clot at room temperature for 30 min).
A peripheral blood sample (2 ml) was collected for each patient (Supplementary Methods online).
Blood (~ 1 mL) was collected by heart puncture.
For each experiment performed, 1.5 mL was collected on the first and last day of culturing.
A blood sample (5 mL) was collected from the decubital vein in EDTA-containing tubes for P-5-HT.
The receptor solution was stirred at room temperature and at suitable time intervals the releasing medium (5.00 mL) was collected.
In the first two studies [83, 84], a spot urine sample (~50 ml) was collected from each subject.
Peripheral venous blood (1 3 mL) was collected and whole blood genomic DNA was extracted and stored at −20 °C.
Umbilical cord blood (30 60 mL) was collected at delivery.
For fractions 1 3, ∼1 mL was collected per fraction, while for the polysome enriched fractions 4 12, ∼0.5 mL was collected per fraction.
Venous EDTA-blood (180 ml) was collected and left to sediment in 6% dextran.
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