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Exact(25)
Up to 5 ml of egg white was removed using a syringe.
1 ml of egg buffer was added to the chamber coverglass.
Values of the test variables [ethanol % (v/v) extraction and pH and volume (mL) of 25 mM FeCl3/100 mL of egg white] were kept according to CCD.
Increasing the volume (mL) of 25 mM FeCl3/50 mL of egg white above 0.55 mL has negative effect on ovotransferrin yield.
(Culture 2.0AA was derived from an initial mixture of 2.0 ml of egg tissue suspension and 1.5 ml of TNMFH, a 4 3 dilution as described above).
3 ml of egg buffer + cells were drawn into a 3 cc syringe and the suspension filtered with a 5 μm Durapore syringe filter.
Similar(35)
The two batches of 5 mL of eggs were fertilized with 5 μl of pooled semen.
Two batches of 5 mL of eggs (approximately 100 to 200 eggs per batch) were used for fertilization.
The eggs were pooled by deme using an equal volume (100 ml) of eggs from each female.
DMSO (10 μL) or egg water solution (50 μL) was added to 1 mL aliquots of egg water.
Proteins were purified as described in [34] starting with 8 ml of Xenopus egg extracts.
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