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The best conditions for the dispersive liquid liquid microextraction were 100 μL of CHCl3 in 1 mL of acetone.
Under optimum conditions, 5 mL of wine were extracted with an extraction mixture consisting of 1.43 mL of acetone, and 173 μL of chloroform at room temperature.
The final optimized method consisted of shaking previously wet soil samples for 30 min with 30 ml of acetone acidified with 1% acetic acid.
As a result, flat response surfaces were obtained and the most convenient conditions were 45% of ultrasound amplitude, 120 s of extraction time and 5 mL of acetone.
Then 3 mL of acetone was added.
The extraction was carried out respectively with 100 and 50 mL of acetone.
Then, to all tubes, 1 mL of acetone was added for deproteination followed by vortex mixing.
The catalyst was washed with 3 ml of acetone and dried at 100 °C.
A 10 mL of acetone was added to the concentrated solution and concentrated/evaporated to dryness.
After becoming clear and homogeneous, added 15 ml of acetone dropwise with stirring for 30 min.
Then 10 ml of acetone was added to the suspension to stop FDA hydrolysis.
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