Sentence examples for ml of embryo from inspiring English sources

0.1 ml of embryo were homogenized with 0.2 ml of lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 1 mM DTT, 1 mM PMSF).

Larvae were anaesthetised in 0.01% 3-amino benzoic acidethylester (Tricaine), positioned on 35-mm glass-bottomed dishes (WillCo-dish®), immobilised in 0.8% low-melting-point agarose and covered with 2 ml of embryo water containing Tricaine.

The 10 µl working stock of DiI solution was added to 96 hpf zebrafish embryos in 4 ml of embryo medium for 15 min. For viewing, fish were anesthetized in tricaine then mounted in 3% methylcellulose with tricaine.

Also, 50-mm Petri dishes containing 4 ml of embryo water were pre-equilibrated in the hypoxia chamber for at least 4 hr prior to embryo transfer because this was determined to be the minimum time necessary to reach <1% oxygen as measured by a dissolved oxygen meter and gave similar viability results when compared with longer pre-equilibration times up to 24 hr (DO-5509; Alfa Electronics).

At stage 30, 2 ml of packed embryo were collected from each cohort and washed three times in 0.1× MMR.

To end hypoxia exposure, plates were removed from the hypoxia chamber, 2 ml of normoxic embryo water was added to each well, and wells were kept open to the ambient air for 10 min prior to replacement of lids and return to the normoxic 28.5° incubator.

Each chick in group 1 was intranasally inoculated with 0.1 mL of 105.5 median embryo infectious doses of strain ck/CH/LDL/091022 [ 14] at 15 days of age.

After 28 hours at 20°C, 50 paa-1 RNAi) paa-1 RNAi worms were transferred to 1 ml of M9 and embryos were isolated as descontrolpreviously (Hannak et al., 2002).

Cohorts of 20 zebrafish embryos were put into a volume of 1 ml embryo medium in 2 ml Eppendorf cups.

Embryos displayed a range of abnormalities in response to MeHg, particularly in brain development, which is influenced by both MeHg concentration and the number of embryos per ml of exposure solution.

To make artificial egg masses, a 2% solution of agarose and seawater was cooled with stirring to 30°C. 10 ml of a known concentration of embryos at the 2 4-cell stage of development was added to 2 4-cell 2% agarostagelutiof to make a solution at a final concentration of 1.5% agarose andevelopment µl−1.