Sentence examples for mixture with final from inspiring English sources

The optimized reaction was undertaken in a 20 μl reaction mixture with final concentrations of 1X LightCycler FastStart DNA master hybridization probes mix, 3 mM MgCl2, 0.5 μM each primer, 0.1 μM probe, and 5 μl of extracted template DNA.

The optimized IS481 MB assay was performed in a 20 μl of reaction mixture with final concentrations of 1X LightCycler™ FastStart DNA master hybridization probes mix, 3 mM MgCl2, 0.1 μM of each primer, 0.3 μM of the IS481 molecular beacon and 5 μl of extracted template DNA.

Then 200 μL of serum and virus mixture with final dilutions of 1 10 to 1 5,120 was added to each of the 6-well plates containing a monolayer of confluent Vero cells (2 × 10 cells/mL in 2% MEM).

With GSBP-PEG as an example, GST (10 μL, 0.8 mM, 18.64 mg/mL in pH 7.4 D-PBS) and GSBP-PEG (10 μL, 8 mM, 40 mg/mL in Milli-Q H2O) were combined to prepare a 20 μL mixture with final concentration of 0.4 mM GST and 4 mM GSBP-PEG.

Real-time PCR was performed in a 20 μl reaction mixture with final concentrations of 1X LightCycler™ FastStart DNA master hybridization probes mix (Roche Diagnostics), 3 mM MgCl2, 0.5 μM of each primer, 0.2 μM of each hybridization probe and 5 μl of extracted template DNA.

GST (0.94 μL, 100 mg/mL in Milli-Q H2O) and GSH-BP (1 μL, 86 mg/mL in MeOH) were added to Milli-Q H2O (8.06 μL) to prepare a mixture with final concentration of 0.4 mM GST and 20 mM GSH-BP, containing 10% MeOH for GSH-BP solubility.

These micrometer-sized powders were mixed in different ratios to obtain powder mixtures with final alloy compositions of Fe100− xCu x (x = 15, 30, 50, 85).

The 1,3-, 1,5- and 1,8-DNN were determined by differential pulse voltammetry (DPV) with limits of quantification 1.0, 1.0 and 0.3 μmol l−1 in concentration range up to 100 μmol l−1 in Britton Robinson buffer and methanol mixture (1 1) with final pH 8.2, 12.3 and 8.2, respectively.

Then 0.2 M of NaOH was dropped into the above mixture with the final pH value of 14.

Methanol was added to the mark to obtain a working standard mixture with a final concentration of 0.8 mg/mL for each analyte.

The reaction mixture with a final volume of 50 μl had 100 ng of DNA template, 1× buffer (Tris-HCl 100 mM, KCl 500 mM), 3.0 mM of MgCl2, 0.2 mM of each dNTP, 0.3 μM of each primer, and 1 U of Taq polymerase.