Sentence examples for compound with final from inspiring English sources

The 16-point curves were prepared as 3× serial dilutions for each compound with final concentrations ranging from 10 μM to 0.003 nM.

The assay was performed at RT in 384-well plates in a final reaction volume of 50 μL in reaction buffer (50 mM Tris pH 7.5, 10 mM MgCl2, 2% v/v glycerol, 1 mM dithiothreitol, 0.1 mg mL 1 bovine serum albumin, 1 unit mL 1 E. coli pyrophosphatase) supplemented with 30 μM UTP, 100 μM GlcNAc-1-P, 0.5 nM recombinant TbUAP, and 30 μM test compound with a final concentration 1% DMSO.

Standards ranged from 64 0.03 μg/mL, and the compounds from 128 0.06 μg/mL with final volumes of 50 μL per well.

The final dose injected into each animal carried about 6.0 mg of the final compound with an activity of 100 150 μCi (Gd: ca. 0.027 mmol/kg; c RGDyK): 2.0 μmol).

Comparison of the 500 MHz H and P NMR spectra of the final compound with those published by others (Gordon et al, 2006) revealed good correlation of chemical shifts and coupling patterns.

After dissolved with 100 μL of dimethyl sulfoxide (DMSO), compounds were stored at -20°C with final concentrations of 5 mg/ml.

The radiochemical synthesis of both compounds was completed within 60 min with final high-performance liquid chromatography purification included.

For inhibition of VASP phosphorylation, cells were treated with AMPK inhibitor, Compound C (final concentration of 5 uM for 5 hr) or PKA/PKG inhibitor, KT5823 (1 uM, 4 hr) and for AMPK activation, 25 uM AICAR was used for 16 hr.

Sperm were adjusted to a concentration of 1.25 × 10 cells per ml with TYH buffer containing different compounds with the following final concentrations: 25 mM NaHCO3, 1 mM N,2′-O-dibutyryladenosine-3′,5′-cyclic monophosphate (db-cAMP; Sigma-Aldrich), 0.5 mM IBMX (Sigma-Aldrich).

This gave wells with final concentrations of 1.95- 2000 μg/mL of the compound.

Compounds were prepared in 15 concentrations with 2.236-fold 2.236-folditrations in dilutiontitrationsoncentratinns ranging from 0.11 pM to 92 μM.