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After reaching 30 mM, 4-mM steps (ten steps in total) were added to make a solution with final concentration of 66 mM.
Make the 10X TMD solution with final concentrations of 500mM Tris pH 7.5, 100mM MgCl2, and 50mM DTT.
Crystallization drops contained equal amounts of protein and precipitant solution with final drop volumes of 1 μL.
For VNO imaging experiment, lyophilized fractions were dissolved in Ringer's solution with final concentration correspond to 1/10 of the original urine sample used.
Then, the concentrated samples were diluted 1 2 v/ v in agar, fixed for 3 h at room temperature in a solution with final concentration 2.5% glutaraldehyde in 0.15 M Na cacodylate pH 7.2, followed by the post-fixation for 1 h at room temperature in a solution with final concentration 1% osmium in 0.15 M Na cacodylate at pH 7.2.
Briefly, the lentil globulins (4%, w/v) were resuspended in stimulate saliva solution with final concentration 7 mM NaHCO3 and 0.35 mM NaCl, pH 6.75, and stirred for 5 min at 37°C in darkness.
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The appropriate amount of distilled water was then added to each vial to obtain polymeric aqueous solutions with final concentration of 2.24 × 10−4 224 mg/L.
There exists a positive solution λ with final eigenvector after using power method based Perron–Frobenius theorem [84].
The final material, meso-tetra(2,6-dichloro-3-sulfonatophenyl porphyrin (H2,6-dichloro-3-sulfonatophenyl porphyrinol–acetone solution with a final reaction yield of 75%.
The data may be distributed at different sites for which a distributed clustering solution with a final merging of partitions is a natural fit.
A CaCl2 solution with a final concentration of 2% (w/v) was prepared in a separate beaker.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com