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DNA preparations, nucleosome reconstitution solution and DNA-TBP-TFIIB reaction mixture were diluted in different buffers according to different experimental protocols.
Stock solutions of PCB118, PCB153, o,p'-DDT and p,p'-DDE, either alone or in mixture, were diluted to a final concentration of 50 nM in 13 mL HPLC mobile phase (90%% A and 10 % B) in glass tubes.
The withdrawn aliquots of digestion mixture were diluted 18-fold with deionized water into sealed 2.0-mL HPLC vials, and then immersed for 10 min in a boiling water bath to terminate the enzyme reactions.
The withdrawn aliquots of digestion mixture were diluted 18-fold with deionized water in sealed 2.0-mL HPLC vials, and then immersed for 10 min in a boiling water bath to terminate the enzyme reactions.
To characterize oxidized products, 100 μL of each reaction mixture were diluted in 900 μL of MilliQ-water, and diluted samples were purified and fractionated to neutral and acidic oligosaccharides using a Hypersep porous graphitized carbon column (Thermo Scientific, MA, USA), following the protocols of Packer et al. [ 31] and Chong et al. [ 32] with modifications.
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The resultant mixture was diluted ×100 and 5 µL of the diluted extract was used for polymerase chain reaction (PCR).
After completion, the mixture was diluted by Et2O.
Total mixture was diluted to 25 mL with distilled water.
The resulting mixture was diluted with water (20 mL) and extracted with dichloromethane (3 × 20 mL).
The mixture was diluted with EtOAc and washed with H2O and 1 M NaHCO3.
The reaction mixture was diluted with Et2O (5 mL) and washed with an aqueous solution of 1M NaHCO3.
Related(20)
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