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Fifteen microliters of enzyme solution were diluted with 2.5 mL of dilution buffer.
Firstly, an ethanol solution of RhoB was prepared (2.25 μmol L-1) and aliquots of this solution were diluted with ACN in volumetric flasks.
Appropriate volumes of the filtered solution were diluted with the mobile phase to obtain the desired concentrations such as 5.0 ppm.
The SWCNTs dispersed in each surfactant solution were diluted to an absorbance (1-mm path length) of 1 at 220 nm using 1 wt% SDS.
Appropriate amounts of the 12.4 µM or 29 µM EAK16-II stock solution were diluted to the desired concentrations (1 12 µM) for the experiments to measure the peptide surface coverage on mica and HOPG surfaces.
The effect of reduction on lipoplex stability was examined on DOPC/DOPE/SS14 (16.6∶33.3∶50 molar ratio) complexed at CR5 by measuring the ability of GSH to restore the fluorescence of DNA/SYBR Green I. Five µl of lipoplexes containing 0.1 µg of pEGFP-N1 in SYBR Green I solution were diluted 1∶20 in 10 mM aqueous solution of either GSH or GSSG.
Similar(43)
The solution was diluted several times.
After dissolution, the prepared solution was diluted 1 4 before use.
The mixture solution was diluted by 100 mL H2O.
The solution was diluted with 20 mL of deionised water.
The QD solution was diluted 30 times with toluene.
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