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To fabricate hydrogel villi structures using a hydrogel mixture, we used a replica molding technique without complicated or expensive fabrication processes.
To analyze this complex mixture, we used sequential native immunoprecipitations, due to the property of PC and SBP precipitations to be eluted in native conditions [27].
For maintenance of the hypoxic gas mixture, we used an incubator with two air sensors, one for CO2 and the other for O2; the O2 concentration was achieved and maintained using delivery of nitrogen gas (N2) generated from a liquid nitrogen tank.
As not only the quality of components but also their relative concentration is an important feature of a mixture, we used different concentrations and recorded full dose response curves.
To shed light on the influence of an IMP on the self-assembly of a lipid/detergent mixture, we used ITC to monitor the reconstitution of KcsA from an OG-solubilized state into proteoliposomes and compared it with the transformation of protein-free micelles into bilayer vesicles.
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To identify common mixtures, we used correlation analyses and exploratory factor analysis.
To study growth and promoter activity in sugar mixtures we used a robotic assay with fluorescence reporter strains.
Describing each of the mixtures performed, based on the knowledge of the properties of the pure components that make up the (theoretical) mixtures, we use the additivity rule with regard to a physical property: P mt T = x a × P a T + 1 − x a × P d T, Open image in new window.
In order to compare the mixture effects with the predictions of CA for a wide range of different VTG levels in the final mixture experiment, we used a "fixed-ratio" mixture design: a master stock was prepared, containing each of the chemicals at their EC50 concentrations.
For the computation of Gaussian mixture model, we used Mclust, an R package which uses the EM algorithm for mixture modeling and the BIC criteria for model count determination (Fraley and Raftery, 1999).
Together, this confirms that the fluorescent phospholipid mixture that we used enabled quantitative evaluation of drug-induced PLD.
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