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To this mixture, we added 1 µl of the serially diluted cDNA prepared from tissue.
To this mixture, we added 1 μg of total RNA.
Briefly, to the 20 μL PCR reaction mixture we added 5.5 μL H2O (RNase, DNase free), 3 μL buffer, 1 μL restriction enzyme, and 0.5 μL BSA.
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Then, we added the mixture to each of the three PrP fibrillar forms.
To determine whether the hybridization intensity at low temperatures can be increased by disrupting the tmRNA secondary structure, we added a mixture of six chaperone oligonucleotides in equal concentrations to the tmRNA solution before hybridization.
It's described almost Biblically on the menu: "When boil is ready, we add our mixture of little lamb special sauce.
If we add the mixture percentages to these two control parameters, we have the three parameters to be controlled and optimised in combustion for generating heat using mixtures of fat and diesel.
Finally, the remaining mixture was added.
The mixture was added onto MDCK cells.
Strain the mixture and add the cream.
Make a well in the mixture and add the milk.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com