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To ensure equimolar amounts of viral RNAs from both samples in the final mixture, we set up several mixtures with varying amounts of each sample that were subjected to the same RT and PCR-amplification procedures for the E1-E2 region described above.
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To rule out the possibility that the SG cells were "sticky," we set up mixtures of MG- and SG-derived trypanosomes in all possible combinations (Table S1B).
We apply both methods to the same mixture data set of varying contributor weights and DNA quantities.
Dredge sweetbreads in mixture and set aside.
Bake until mixture is set and dry, about 5 minutes.
Stir into the yeast mixture, and set aside. 3.
3 Reserve around 170g of the mixture and set aside.
The mixture will set at 20C, but refrigeration speeds up the process.
Place in the refrigerator for a few hours to allow the seed mixture to set.
3. Rub the pork all over with the spice mixture and set in a roasting pan.
Scoop up the mixture and set it in the middle of a large sheet of clingfilm.
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