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After a denaturation step (NaOH 0.5 M during 5 s) and a wash step for the removal of unlabeled complementary DNA of the amplicon, the biotinylated single-stranded DNA present on the streptavidin-coated Sepharose beads were transferred to a 96-well plate and used as template for the pyrosequencing assay with a mixture of sequencing primers.
The set of input reads contain a mixture of sequencing technologies including Illumina, 454 and Sanger.
These problems may be overcome by hybrid sequencing strategies using a mixture of sequencing methodologies, by new assemblers, and by sequencing more deeply.
We have developed a simulation approach to help determine the optimal mixture of sequencing methods for most complete and cost effective transcriptome sequencing.
The latter categories may represent a mixture of sequencing, alignment and variant calling error, deviations from homozygosity, triallelism and paralog incorporation.
Contrary to on-target exome data, off-target reads are unintentional by-products of the enrichment technologies and are thus subject to a mixture of sequencing biases that are highly variable and dependent on genomic context.
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The complete set of Figures, shown in Supplementary Figure S1, and the associated summary statistics provide information about the potential mixture of sequences found in each OTU.
When amplification is initiated from a single molecule any base change occurring during the first cycle of duplication results in a 50∶50 mixture of sequences and any base change during the second cycle of duplication results in a 75∶25 mixture of sequences.
Here, we describe how the presence of a low-diversity mixture of sequences during the cluster calling cycles interferes with the mapping of cluster coordinates, and can result in a significant loss of data.
In each subject V3 amino acid residues revealed a mixture of sequences with low or high net charge predicting, as confirmed by two independent algorithms, CCR5 or CXCR4 coreceptor use respectively [39] [41].
Of the remaining multi-sequence macrofossils, four had a mixture of "correct" sequences and sequences of other species and one had a mixture of sequences from other species.
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mixture of timing
mixing of sequencing
solution of sequencing
mixture of order
mixture of sequenced
mixture of footage
mixture of sequences
mixture of urbane
mixture of invulnerability
mixture of light
mixture of desperation
mixture of cash
mixture of charisma
mixture of community
mixture of baroque
mixture of speech
mixture of shock
mixture of concrete
mixture of melody
mixture of method
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