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The complete set of Figures, shown in Supplementary Figure S1, and the associated summary statistics provide information about the potential mixture of sequences found in each OTU.
When amplification is initiated from a single molecule any base change occurring during the first cycle of duplication results in a 50∶50 mixture of sequences and any base change during the second cycle of duplication results in a 75∶25 mixture of sequences.
Here, we describe how the presence of a low-diversity mixture of sequences during the cluster calling cycles interferes with the mapping of cluster coordinates, and can result in a significant loss of data.
In each subject V3 amino acid residues revealed a mixture of sequences with low or high net charge predicting, as confirmed by two independent algorithms, CCR5 or CXCR4 coreceptor use respectively [39] [41].
We chose gDNA because it is a particularly complex mixture of sequences and provides a stringent test for our assay.
The aDNA sequences endogenous to individual macrofossils might be probabilistically distinguished from exogenous sequences; endogenous DNA dominated the mixture of sequences in most macrofossils.
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After a denaturation step (NaOH 0.5 M during 5 s) and a wash step for the removal of unlabeled complementary DNA of the amplicon, the biotinylated single-stranded DNA present on the streptavidin-coated Sepharose beads were transferred to a 96-well plate and used as template for the pyrosequencing assay with a mixture of sequencing primers.
The set of input reads contain a mixture of sequencing technologies including Illumina, 454 and Sanger.
The UGA10 clade contained a mixture of sequence types and was most similar to the IC11-clade.
These problems may be overcome by hybrid sequencing strategies using a mixture of sequencing methodologies, by new assemblers, and by sequencing more deeply.
We have developed a simulation approach to help determine the optimal mixture of sequencing methods for most complete and cost effective transcriptome sequencing.
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