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The heteromers were formed by mixing equal molar of homomers and incubated at 37°C as described previously (Wang et al., 2011a).

All probes were prepared based on the MCPC rule, i.e., multicolor probes were prepared by individually labeling the same probe with different fluorescent colors followed by mixing equal molar quantity of each colored probe to produce a target-specific multicolored probe set.

Selections were performed by mixing equal molar concentrations of 1 kb, 2 kb, and 4 kb insert sized genomic libraries within the host strain P. aeruginosa PAO2003, allowing those mixtures to recover in non-selective conditions for 1 hour, and then spreading an equal numbers of cells onto plates containing increasing concentrations of gentamicin, tobramycin, or amikacin.

2-24a/Cu was freshly prepared by mixing equal molar ratios of 2-24a and CuCl2, and diluted to the appropriate concentrations before treatment.

The second round of PCR was performed to produce the full-length AB'P, using the primers A-up and P-down and mixing equal molar amounts of the first-round products as template.

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An alternative but more practical way is to mix equal molar quantities of probes each labeled with a single but different colors to function as a single probe set labeled with different colors.

We mixed equal molar concentrations of the insert and vector fragments for multi-way CPEC.

Create a roux by mixing equal parts butter and flour.

Binary (mini-chaperone/DsbA-, mini-chaperone/hPPIase- and DsbA/hPPIase-Sepharose) and ternary (mini-chaperone/DsbA/hPPIase-Sepharose) refolding matrices were prepared by thoroughly mixing in equal molar ratios of the single refolding gel (mini-chaperone-Sepharose, DsbA-Sepharose and hPPIase-Sepharose).

DNAM and DDEDA were pre-mixed at equal molar concentrations, and were further mixed with the indicated candidate biotin derivatives to prepare Mixture A, B and C in methanol, for the average of molar quantities of biotin derivatives equal to that of DDEDA.

In order to solidify the seed layer, the substrate was heated in air at a constant temperature of 250°C for 30 min. In the growth method, zinc nitrate hexahydrate [Zn(NO3 2⋅6H2O] (99.998%) and hexamethylenetetramine (C6H12N4) (99.998%) were mixed with equal molar concentration in DI water and kept under continuous magnetic stirring for 30 min to get a uniform growth solution.

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