Sentence examples for combining equal molar from inspiring English sources

Prior to adaptor ligation, we carried out an adaptor Y fork annealing reaction by combining equal molar ratios of methylated PE1 and methylated PE2 adaptors.

Samples were prepared by combining equal molar amounts of the P. glauca RNAs isolated from secondary xylem (juvenile trees) and phelloderm.

In vitro quenching of actin assembly and disassembly was accomplished by combining equal volumes of the bead motility reaction and 37.5 µM Latrunculin B-phalloidin (1 1, therefore 5 molar excess relative to concentration of actin) diluted in buffer containing 20 mM HEPES [pH 7], 100 mM KCl, 100 µg/ml BSA, 0.5 mM TCEP.

Amplicons were gel purified and quantified, then combined in equal molar concentrations.

Resulting mRNA was quality verified as above and combined in equal molar ratios across tissues.

To assemble the respective duplexes, the complementary strand 5′-d CTTCTTGTCCG -3′ was combined with equal molar quantities of either 5′-d CTTCTTGTCCG -3′ 5′-d CTTCTTGTCCG -3′′, or 5′-d(CGGAC SAGAAG)-3′, heated to 75 °C for 15 min, and cooled to room temperature to form the unmodified, and R,R- and S,S-duplexes, respectively.

Ligated products from multiple species were quantified simultaneously with a Quant-iT Picogreen dsDNA Assay Kit and the Qubit Fluorometer (Life Technologies) to combine them at a nearly equal molar ratio.

The HSP-based anti-tumour vaccines were generated by incubating and non-covalently complexing recombinant proteins; hsp110 was combined with gp100 or CA9 at an equal molar ratio as previously described (Wang et al, 2003, 2008).

Compound combinations were prepared by mixing the four compounds with equal molar ratio.

All proteins were equally pure and soluble, and they were used at equal molar amounts (Figure 6A).

Solvents with equal molar fractions of propane were compared.