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Polyacrylamide was prepared by mixing equal volume of acrylamide and potassium persulfate.
Crystallization trials were carried out at 18°C by mixing equal volume of protein and reservoir solution using the sitting-drop vapor diffusion method.
Combining two or three materials by mixing (equal volume) or by applying them in layers (equal layer thickness) gave similar effluent qualities.
By mixing equal volume of SPADNS and zirconium-oxychloride solution, the SPADNS reagent is prepared which is used in the experiments.
The CUPRAC reagent was prepared by mixing equal volume of 1 mM CuCl2*2H2O (in methanol/water 50/50), 25 mM Sodium acetate (in acetone/water 25/75), and 2 mM neocuproine (in methanol/water 50/50).
The final purified P protein was concentrated to 10 mg/mL and crystallized with hanging drop vapor diffusion method by mixing equal volume of P protein with reservoir solution containing 0.1 mol/L LiCl, 18% (w/v) PEG 3350 and 10% (v/v) 2-Methyl-2,4-Pentanediol (MPD) at 16°C.
Similar(49)
Fe0.7Co0.3 alloy nanoparticles were prepared by mixing equal volumes of ME1 and ME2 containing metal salts and precipitating agent, respectively.
Crystals were successfully grown at 18°C using the sitting drop vapor diffusion method by mixing equal volumes of protein and reservoir solution.
To assess the ABTS free radical scavenging stock solution was prepared by mixing equal volumes of 7 mM ABTS solution and 2.45 mM potassium persulfate solution [40].
In this experiment, the complexes were first prepared at several charge ratios in PBS, by mixing equal volumes of dendrimer and asODN solutions.
Sperm viability was assessed microscopically from smears that were prepared by mixing equal volumes of eosin-nigrosin stain with epididymal suspensions, incubation for 30 s, distributing the stained suspensions on glass slides, and drying in air.
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