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The flowability of the mixed samples was tested immediately after mixing following ASTM D6103 (2004).
This series of mixed samples was analyzed using the MRM method established above.
In these experiments, successful assembly of mixed samples was not dependent on the abundance of repetitive sequences or on the GC content of the genomes (Table S2).
After 30 min, the absorbance of mixed samples was measured at a wavelength of 720 nm.
The data of two mixed samples was used for explorative factor analyses.
The analysis of the pure MCYST-LR calibration and mixed samples was carried out to evaluate the influence of food additives extracts on the quantitative responsivity of the mass spectrometer.
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After 4 h, the mixed samples were stained with PI and analyzed by FACS.
In the natural runoff experiments, volume proportional mixed samples were collected after each runoff period during 1992 1999.
After 4 h, the mixed samples were stained with PI, and the killing of target cells were analyzed by FACS.
The mixed samples were then extracted with 1.5 mL of dichloromethane: n-Hexane (1 2), by vortex mixing for 2 min.
In order to dissolve the PMMA powders, the mixed samples were placed in a dark room for several days.
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