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The most significant single locus result in maternal combined samples was in prostaglandin E receptor 3 (PTable) (Table 4).

The joint analysis of all combined samples was performed using logistic regression with gender, age and sample cohorts as covariates.

In the first round of enrichments, 1 g of the combined samples was inoculated into 100 ml of MM containing 157.5 μM GGE as the sole carbon source.

Thereafter, each of combined samples was fractionated into 6 fractions by strong cation exchange (SCX) chromatography according to the manual of the manufacturer (AB Sciex).

The final association analysis of the combined samples was performed by using the matched GWAS dataset of 706 cases and 514 controls and the two independent validation series, consisting a total of 3148 cases and 7843 controls (this number excludes those extended samples used as controls with reasons as previously explained (13)).

The gas bearing coal-sandstone combined samples were subjected to unloading tests, including unloading confining pressure (UCP) under constant axial tests and UCP-reloading axial stress (UCP-RAS) tests.

However, studies using multivariate analysis on combined samples are rare and further investigations using this approach are warranted [31].

The most significant associations in the combined samples were from two major PTB pathways: 1) prostaglandin (PG) effectors (PTGER3) in maternal samples that influence PG mediated uterotonic activity and 2) decidual hemorrhage (PON1) in fetal samples.

Combined samples were fractionated by preparative isoelectric focusing using Free Flow Electrophoresis (FFE) as described [24], and the peptide fractions analyzed by µLC-MS/MS on a linear ion trap instrument.

The combined samples were quantile normalized.

The combined samples were applied to 24 cm immobilized pH gradient (IPG) strips (BioRad) with a pH 3 10 linear gradient.