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The aspecific turbidity of MPN tubes from control and artificially contaminated samples was frequent above the theoretical inoculum of 10 CFU g−1.
The accuracy of the developed method for the analysis of naturally contaminated samples was tested by participating in a proficiency test organised by BfR with tomato juice samples containing Alternaria toxins at levels from 1.56 to 53.0 µg kg−1.
The number of different OTUs only detected in contaminated samples was significantly higher than the number of OTUs only detected in non-contaminated samples.
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CSF was visually inspected for the presence of blood contamination and contaminated samples were excluded from further analysis (5 CSF samples from LC-MS/MS analysis and 4 samples from WB analysis) leaving samples from 27 animals for LC-MS/MS analysis and 36 animals for WB analysis.
Brains were removed after in situ perfusion with PBS (pH 7.4 at room temperature) at 1 2 ml/min for 4 5 min. CSF and brain samples were visually inspected for blood contamination and contaminated samples were excluded from exposure analysis and analysis of amyloid-β in CSF.
The contaminated samples were from seven products that had already been withdrawn, including Findus lasagne and Tesco burgers.
The contaminated samples were found outside the reactor building for the first time, causing concern that soil and sea in the surrounding area will be poisoned.
Contaminated samples were also sent to another CDC lab, the report says.
The contaminated samples were then dried at room temperature in a sample holder.
The contaminated samples were tested alone and in the presence of SnO2 and MoO3.
The contaminated samples were prepared by mixing the soils with crude oil in the amount of 2%, 4 %, 8 %, 12 and 16% by dry weight.
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