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The stored heat from the PCM pouch would then have to be discharged by immersing in water for about 15 min to solidify the PCM before re-use.
The graphite was peeled off the HOPG with a Scotch tape; this obtained graphite was first attached to a glass plate coated with a photoresist and heated at 80 °C for 3 min to solidify the photoresist.
The resultant mixture was extruded drop wise into boric acid (3%% v/v) and CaCl2 (2 % w/v) solution using syringe (nozzle diameter 5 mm) needle to form uniform size beads and stirred for 30 50 min to solidify, thoroughly washed with distilled water and store at 4 °C for 24 h [26].
Coated Petri dishes were incubated at 37°C in CO2 incubator for 15 min to solidify.
After filling, the vials were cooled to 4 °C for 20 min to solidify the suspension.
The slides mounted with cells were covered with coverslips and kept in the refrigerator for 3-5 min to solidify the low melting agarose.
Similar(49)
Pre-cooled glass coverslips were coated with 25 µg/ml of quenched fluorescent substrate, DQ-Gelatin (Molecular Probes) in a 2% gelatin/2% sucrose solution in PBS and incubated on ice for 15 mins to solidify.
Test tubes were corked, sterilized (at 121 °C, 1.5 p.s.i, for 30 min) and allowed to solidify in a slant position.
The plates and chamber slides were left at 37°C for 30 min for Matrigel to solidify.
Geltrex (Invitrogen, Carlsbad, CA) was thawed on ice and 40 μL was pipetted per well and left for 30 min at 37°C to solidify.
Then, this medium was moderately cooled at 28 °C for 20 min and poured in sterilized Petri dishes and left for 20 30 min to be solidified for further utilization in downstream process.
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