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Typical etching times were 30 min to remove the resist.
The homogenates were centrifuged at 1 000×g for 10 min to remove nuclei and at 10 000×g for 15 min to remove mitochondria and synaptosomes.
The cells were pre-plated on plastic tissue culture dishes (30 min) to remove non-cardiomyocytes.
The homogenate was centrifuged at 1000g for 10 min to remove the nuclear fraction.
Samples were removed and centrifuged at 30,000 g for 30 min to remove large aggregates.
The homogenate was centrifuged at 800 g for 15 min to remove unbroken cells.
After sonication homogenized tissue was centrifuged at 1500 xg for 10 min to remove cell debris.
After incubation, the culture was centrifuged at 15000g for 20 min to remove cells.
Homogenates were then centrifuged (12,000×g, 10 min) to remove the yolk.
Briefly, samples were treated with DNase I for 25 min to remove any contaminating DNA.
Samples were centrifuged at 10,000 rpm for 10 min to remove particulates.
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