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Exact(28)
Mice had 5 min to retrieve all pellets.
Paraffin-embedded tissue samples were sectioned, deparaffinized, and then pretreated by autoclaving in 10 mM citric acid buffer for 15 min to retrieve antigenicity.
Although all Asic3+/+and Asic3+/− mice retrieved three pups in 15 min, only 58.8% Asic3−/− mice retrieved three pups, and most took more than 20 min to retrieve all pups.
The sections were deparaffinized in xylene and dehydrated in graded ethanol continuously, and pre-treated with citrate buffer (pH 6.0) at 100°C for 20 min to retrieve the antigen.
Briefly, sections were deparaffinaged, rehydrated to distilled water, and immersed in boiling citrate buffer for 10 min to retrieve antigen.
Formalin-fixed sections were boiled in 10 mM Na-citrate pH 6.0 for 10 min to retrieve antigens.
Similar(32)
Endogenous peroxidase activity was blocked by immersing sections in 0.3% H2O2 (Sigma, H1009) diluted in methanol for 30 min. To retrieve antigens, sections were boiled in 0.01 M citrate (pH 6.0) or 0.01 M EDTA buffer (pH 8.0).
The lung sections were deparaffinised and rehydrated, then microwaved in 0.01 M sodium citrate buffer for 15 min at 90°C to retrieve epitopes, and cooled at room temperature.
Paraffin-embedded tumour sections were dewaxed in xylene and ethanol, and autoclaved for 15 min in an antigen retrieval solution to retrieve their antigen epitopes; endogenous peroxidase activity was blocked by 3% hydrogen peroxide.
18 min: Eccleston does well to retrieve a long ball over the top. he plays it back to Cole on the left, and the ensuing cross comes to nothing.
The barrier was then removed and each dam was scored for the total number of pups retrieved in 10 min and the latency to retrieve the first and all of the pups back to the nest (39).
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