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The mixture was swirled and allowed to stand at room temperature for 10 min to enable the hydrazine to penetrate the matrix.

The samples to be observed under the SEM were mounted on a conductive carbon imprint left by the adhesive tape prepared by placing the samples on the circular holder and coated for 5 min to enable it conduct electricity.

HDPE/ND nanocomposites were fabricated via a melt-blending method using a Thermo Haake mixer (Thermo Scientific, Boston, USA) with a rotation speed of 60 rpm at 180 °C for 15 min to enable the in situ reaction complete.

Locomotor activity was measured for 30 min to enable comparison to the conditioning sessions.

Beginning on the third day, to the termination of the experiment, colony entrances were blocked with wire-mesh for 15 min intervals separated by at least 30 min to enable the capture of returning focal foragers.

Conditions for the real-time PCR were established empirically and included incubation at 50°C for 2 min to enable uracil-DNA-glycosylase (in Master Mix) to act on samples and 94°C for 10 min to activate AmpliTaq Gold polymerase, followed by 40 cycles of 15 s of denaturation at 94°C and 1 min of annealing and extension at 60°C.

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Similar(45)

After 15 min incubation to enable partitioning, 1 ml was removed from the aqueous layer and the OD440 recorded.

Beverages were given to the subject in an opaque bottle, and they were asked to consume it within 5 min. To enable comparisons, the same methods for the assessment of endothelial function were used as in the previous study (14).

MVPA using the 2,020 counts/minute threshold was analyzed in terms of both total accumulated time and bouts of at least 10 consecutive minutes (MVPA10-min bouts) to enable comparison with physical activity recommendations in the United Kingdom, the United States, and elsewhere which stipulate that the achievement of health benefits requires undertaking MVPA in sustained bouts (39– 41).

After each set of four laps, a break of approximately 5 min was introduced to enable the participant to recover sufficiently.

Use of the process for 15 min was insufficient to enable successful isolation of analytes from hair structure, both for simultaneous and separate hydrolysis and extraction.

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