Sentence examples for min before counterstaining from inspiring English sources

After additional washing in TBS for 20 min, DAB was added to each slide (100 µl/slide) for 5 min. Slides were then washed in distilled water for 5 min before counterstaining in Mayer's haematoxylin for 15 seconds.

All staining was completed using diaminobenzidene (EnVision HRP kit, Dako, Cambridge, USA) for 2 3 min before counterstaining with haematoxylin.

Slides were then rinsed in water for several min before counterstaining with Gills III Hematoxylin (Poly Scientific).

Staining was revealed by development in the chromogen 3,3-diaminobenzidine tetrahydrochloride (DAB) for 5 10 min before counterstaining with haematoxylin in preparation for mounting.

Staining was revealed by development in the chromogen 3,3-diaminobenzidine tetrahydrochloride for 5 10 min before counterstaining with haematoxylin before mounting.

Slides were then treated with one drop of COPPER (4 min) before counterstaining with hematoxylin (4 min), followed by bluing agent and finally rinsed with reaction buffer.

for 30 min at room temperature before counterstaining with by 4'-6-Diamidino-2-phenylindole (DAPI) (Sigma).

Sections were incubated with the TUNEL reagent (Roche) at 37°C for 30 min before being counterstained with DAPI.

Primary staining against luciferase was done with 1∶5000 to 1∶10000 diluted Novus goat polyclonal anti-firefly luciferase antibody (NB100-677) in Dako Antibody diluent for 1 hour followed by secondary staining with 1∶500 diluted rabbit anti-goat (Dako) for 30 min. Slides were then treated with Dako envision anti-rabbit for 30 min, and then treated with DAB before counterstaining with hematoxylin.

Slides were developed with DAKO DAB plus for 5 min followed by DAB Enhancer for 3 minutes before counterstaining with hematoxylin.

For IHC, a biotinylated secondary antibody was added for 30 min followed by an avidin/biotin-based peroxidase solution and incubated with 3,3′-diaminobenzidine solution before counterstaining with haematoxylin.