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Exact(57)
To examine cell migration, migrating cells were imaged at 5 min intervals to generate plots of cell migration distance over time.
In our Boyden migration assay serum containing media was used as a chemoattractant and PMA was added as a positive control to demonstrate poorly migrating cells were competent for migration.
After 24 h, non-migrating cells were scraped away and migrating cells were stained with Diff Stain (IMEB, San Marcos, CA, USA).
Migrating cells were stained blue.
Additionally, the morphology and spreading of migrating cells were also assessed.
Migrating cells were evaluated by reading at different time points the lower and the upper sides of the membrane with the Spectrafluor reader (Tecan).
This was not surprising because our results showed that after TGF-α treatment most of the actively migrating cells were EGFr negative neuroblasts.
Migrating cells were stained in Mayer's haematoxylin.
Similar(3)
The Transwell chambers were incubated at 37 °C for 48 h and non-migrating cells were removed by a cotton swab, while the migrated cells were fixed, stained with crystal violet, and counted for ten random fields/chamber.
Non-migrating cells were removed with a cotton swab.
The non-migrating cells were removed from the upper surface by cotton swabs.
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