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Although the number of migrating cells was lower than that in cultures containing proliferating cells, the fraction of migrating cells was similar (<25%).

This increase in migrating cells was partially inhibited when the anti-bFGF antibody was incubated with the cell debris or the lysates.

A random position in the cytoplasm not containing the centrosome in migrating cells was exposed to the laser.

Analysis of migrating cells was carried out by counting the number of cells that passed through the transwell after that period.

For quantitative assessment, the number of stained, migrating cells was then counted under microscopy at five fields per filter in three independent experiments.

The analysis revealed that the distance covered by migrating cells was significantly decreased when TPC-1 cells were exposed to the CGS compared to cells exposed to the vehicle (DMSO) (Fig. 4).

To examine cell migration, migrating cells were imaged at 5 min intervals to generate plots of cell migration distance over time.

In our Boyden migration assay serum containing media was used as a chemoattractant and PMA was added as a positive control to demonstrate poorly migrating cells were competent for migration.

After 24 h, non-migrating cells were scraped away and migrating cells were stained with Diff Stain (IMEB, San Marcos, CA, USA).

Representative photomicrographs of the DAPI-stained nucleus (blue staining) used for quantification of migrating cells are shown in b.

Migrating cells were stained blue.