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In medium the fast migrating band was greatest for heparin plus glucose treatment while the slower migrating bands were greater for the treated group than control.
Moreover, multiple slower migrating bands were apparent upon immunoprecipitation of Gli3R from SUMO1 transfected cells, compatible with the possibility that this region contains more than one SUMOylation site.
Meanwhile, slow migrating bands were also observed for mei4 mRNA produced in pla1-ts37 cells shifted to the restrictive temperature, though their intensity was moderate.
In the in vitro SUMOylation assay, slower migrating bands were also obtained, except for the case of the F-NACC1-K167R F-NACC1-K167R F-NACC1-K167R
When lambda phosphatase was added to immunoprecipitated Maf1.pk the slower migrating bands were absent and an increase in the faster migrating non-phosphorylated Maf1.pk was observed (Fig. 3F).
As shown in Fig. 3E, the slower migrating smeared bands were down-shifted and became a single sharp band for each TFE3 protein, indicating that those slower migrating bands were phosphorylated TFE3 proteins.
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The medium migrating band was susceptible to chondroitinase ABC digestion only, thus confirming the identity of this band as DS.
A faster migrating band was also reproducibly amplified from cDNA of the tumour containing 314A>G (Fig. 4B), compared to control TERT-NHUC cDNA.
Co-migrating bands were assumed to be originated from the same genetic locus.
Slower-migrating bands were undetectable in Noxa-5A, which predominantly localized in the cytosol.
The slowest-migrating bands were heteroduplexes of wild-type and mutated sequences.
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