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SEM images showed that the lamellar body of migrating cells, seeded on 50-nm nanodot arrays, exhibited wide and thick characters with a large number of filopodia (Figure2).
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Migrating cells were fixed 48 72 h after seeding using 4% PFA for 2 h, permeabilized in 0.5 % (vol/vol) Triton-X 100 for 30 min at room temperature, and stained overnight at 4 °C using Alexa Fluor 488 phalloidin.
Migrating cells were counted (10 random fields per insert) and expressed as percentage of migrating cells in relation to the total number of seeded cells.
Nuclei of migrating cells incorporated bromodeoxyuridine.
Between the cells the extracellular matrix – a mesh of proteins, carbohydrates, and other molecules – helps migrating cells find their destination.
Migrating cells were stained blue.
Histograms show the quantification of migrating cells.
Cells seeded on fibronectin gradients preferentially migrate towards areas of higher fibronectin concentration with concurrently higher overall adhesiveness [52].
Cells seeded in inserts were allowed to migrate for 24 hours.
While these cells may have greater propensity to migrate than endogenous chondrocytes, the number of cells seeded is difficult to control because they are likely to attach only loosely to the seeded tissue or clump together, generating only focal connections between tissue surfaces (unpublished observations).
Reductions in migrating cell numbers after i.p.
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