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CFP fluorescence of transfected AAV-293 cells was monitored by fluorescence microscopy to estimate transfection efficiency.
Cells were then fixed for immunolabeling and confocal microscopy to estimate cell volume.
Vero cells were imaged also by conventional wide-field fluorescence microscopy, to estimate the relative intensity of the Golgi fluorescence.
In some experiments cells co-transfected with the GFP plasmid were analyzed by fluorescent microscopy to estimate transfection efficiency, which was always >90%.
To analyze trypanosome within-host dynamics, we established infections by infecting mice with 1000 AnTat1.1 trypanosomes from a donor infection and monitored them daily by microscopy to estimate parasite number.
Recently, two groups have used fluorescence microscopy to estimate the stoichimetry of kinetochore proteins, which led them to conclude that the budding yeast CenH3 (Cse4) is present at a much higher abundance at centromeres than can be explained by the presence of a single centromeric nucleosome (Coffman et al. 2011; Lawrimore et al. 2011).
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Lyophilized, porous composites were examined under scanning electron microscopy (SEM) to estimate the size of the pores, an essential requirement for an ideal scaffold.
We employ high-resolution energy-filtered cross-sectional transmission electron microscopy (EFTEM) to estimate the degree of layer intermixing in a TiAlN/VN low friction, wear-resistant nanoscale multilayers with a periodicity of approximately 4 nm grown by industrial-scale unbalanced magnetron ion-assisted PVD.
Of note, the confocal microscopy enabled to estimate the individual changes of fluorescence occurring in each of the cells selected to test (Fig. 1 A, B), whereas FACS analysis evaluated the average fluorescence per cell (Fig. 1 C).
Digital microscopy is used to estimate tow cross-section and path, and quantify the amount of variation of these parameters.
Despite the small size of the NRs, the imaging by scanning probe microscopy allowed us to estimate the position and place of indentation.
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