In the fall semester, students also sent prepared electron microscopy grids fixed with their purified phage to Lehigh University (Bethlehem, PA), an institution equipped with an electron microscope that is capable of determining phage morphology.
However, visualizing the internal ultrastructure leading to these phenotypes still requires electron microscopy of fixed samples.
To validate the enrichment, the unsorted and sorted cell fractions obtained by FACS was analyzed under fluorescence microscopy, or fixed, embedded, sectioned, and stained as above to determine the percentage of germ cell type.
To test this possibility, we performed electron microscopy of fixed optic axons of NF-L null and DL20J mice, and further morphometric analysis by counting clear ER vesicles in these mutant axons and calculating the density of ER (see methods).
Whole-granule recapture within intact islets has been controversial and to further our analysis we performed electron microscopy on fixed islets.
Where there is a relatively high prevalence of malaria and an established microscopy service, microscopy would usually be less expensive than RDTs because most of the costs associated with microscopy are fixed costs, and microscopy can also be used to diagnose other diseases.
The fractions collected from the size-exclusion chromatography containing the SecA/SecB complex were immediately applied to electron microscopy grids and fixed by negative staining.
Material for scanning electron microscopy was fixed and examined as explained previously [43].
For electron microscopy, we fixed the dissected PSGs and processed the samples as previously reported [55].
Cells grown on 22-mm2 cover slips for conventional fluorescence microscopy were fixed with 4% paraformaldehyde for 12 h at 4°C.
