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The microstructure was analysed by means of optical microscopy and determination of the BET surface and pore size distribution.
A specific characterization protocol was set up, entailing hot-stage microscopy and determination of phase composition and microstructure.
Cardiac myocyte isolation, confocal microscopy and determination of MPT ROS threshold have been described [12], [29].
Enzymatic activities, crystal violet staining, electron microscopy and determination of the sensitivity to stress were performed as previously described [30].
Observation using light sheet microscopy and determination of fluorescence intensity along transverse profile of growing root hairs showed the oscillatory pattern of root hair growth (Additional file 1: Figure S8C,D, Additional file 4: Video S3).
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The PALDPE blends were prepared in a co-rotating twin screw extruder and were characterized by Molau tests, differential scanning calorimetry, scanning electron microscopy, rheology, and determination of the ultimate mechanical properties, including impact tests.
The use of highly reproducible and spatiallyhomogeneous thin film matrices permits automated microscopy and quantitative determination of the response of hundreds of cells in a population.
The new nanosorbent was characterized by Fourier-transform-infrared (FT-IR), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM) and determination of surface area.
Constructs were cultured for 28 days in a rotating bioreactor prior to scanning electron microscopy (SEM), histology, and determination of their water, DNA, glycosaminoglycan (GAG) and collagen II contents.
Apoptosis was determined by fluorescence microscopy and western blot determination of PARP processing.
Samples were shipped on dry ice to our laboratory, and species identification was performed by using light microscopy and following the determination key of Hopkins and Rothschild (9 ).
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