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In this paper we show by confocal microscopy and deconvolution analysis that Prep1 and 4EHP co-localize in the cytosol of growing mouse oocytes, demonstrating their interaction by co-immunoprecipitation and pull-down experiments.
Microscopy and deconvolution were as described above.
To assess the architectural organization of cell colonies in 3D culture, expression and localization of total ezrin, Y477F-ezrin (VSVGtagged)and F-actin were examined using confocal spinning disk microscopy and deconvolution image analysis.
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High-resolution confocal microscopy and image deconvolution allowed us to assess the overlap between axons and dendrites with an accuracy close to the optical diffraction limit (<300 nm).
For deconvolution, image stacks were collected using a DeltaVision Elite microscope and deconvolution was performed by Huygens Professional 4.5.1p3 software.
Systematic dissection of this observation using transduction rescue assays, PCR analyses for vector genomes, western blotting and deconvolution microscopy, studies herein provide compelling evidence that a wide range of cell types are capable of successfully transmitting vector genomes in a process that is scalable (dose-dependent) at the level of vector input.
Intravital microscopy, image deconvolution, and transmission electron microscopy (TEM) indicated that the crawling monocytes extended numerous and mobile filopodia-like structures in contact with the endothelium in the dermal and kidney cortex blood vessels of Cx3cr1 gfp/+ ;Rag2 −/− ;Il2rg −/− mice.
We examined the subcellular location of endogenous Msi1 and Msi2 in MIN6 cells using deconvolution microscopy and found a punctate cytoplasmic localization consistent with a regulation of translation.
We demonstrate that MTRIP-labeled hRSV virions could be isolated on glass coverslips, stained for viral proteins, and interrogated using both diffraction-limited deconvolution microscopy and the subdiffraction resolution method direct stochastic optical reconstruction microscopy (dSTORM).
Differential interference contrast (DIC) and fluorescent images were captured and processed using deconvolution microscopy and a merge of the captured images is presented on the far right pane (merge).
The tissue samples were embedded in ProLong Gold Antifade Reagent with 4',6-diamidino-2-phenylindole (DAPI; Life Technologies), subjected to fluorescence microscopy and analyzed using 2D Deconvolution MetaMorph software (Molecular Devices, http://www.moleculardevices.com/).
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microscopy and electrophysiology
microscopy and chest
microscopy and density
microscopy and cell
microscopy and scanning
microscopy and immunoprecipitation
microscopy and synchrotron
microscopy and culture
microscopy and electrochemistry
microscopy and image
microscopy and laser
microscopy and particle
microscopy and diffraction
microscopy and contact
microscopy and tissue
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