FIB-milled nanochannels are used to measure the effect of nanochannel dimensions on DNA mobility by fluorescence microscopy and to sense DNA translocation by resistive-pulse sensing.
Moreover, Au NPs have shown promises as labelling/contrast agents (transmission electron microscopy/X-rays) and sensing agents.
In this context, we use confocal Raman microscopy and current sensing atomic force microscopy (CS-AFM) to study phonons and the electronic transport in semiconducting SWCNTs, which were aligned between palladium electrodes using dielectrophoresis (Kuzyk Electrophoresis 32 2307 2313, 2011).
The use of a wide range of available methods, techniques, and protocols in molecular biology, electron microscopy, and in situ and remote sensing facilities allow us to study all groups in a better and more systematic way.
To this end we have developed a novel in situ hybridization approach in combination with immunoelectron microscopy, using sense and anti-sense RNA probes that are derived from total cellular or cytoplasmic poly(A+) RNA.
In comparison to many of the microfluidic bioanalysis sensing techniques, such as fluorescence microscopy and electron microscopy, the SERS detection method avoids any fixation, freezing, or staining of the biological samples with exogenous labels.
More recently, other techniques have been used to investigate and characterize EVs including; cryo-EM, nanoparticle tracking analysis (NTA), dynamic light scattering, resistive pulse sensing (IZON qNano), atomic force microscopy and Raman spectroscopy (as reviewed in Refs. 24 26).
Atomic-scale surface imaging in liquids was recently achieved using frequency modulation atomic force microscopy with improved force sensing.
Sections were observed under light microscopy, and the absence of hybridization signal in the sense probe-treated materials was verified.
The morphologies and the electrochemistry of the formed sensing surface were investigated by using scanning electron microscopy and electrochemical techniques including electrochemical impedance spectroscopy and cyclic voltammogram.
