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Over at the National University Medical Institutes, one of the first institutes to implement core services for researchers, specialized microscopists at the Microscopy Center routinely analyze research samples for researchers using the most sophisticated technologies in confocal microscopy and tissue imaging--technologies few individual investigators could afford.
The ability of sNAG treatment to induce defensin expression and bacterial clearance in WT and AKT1−/− mice was carried out using immunofluoresent microscopy and tissue gram staining.
R.L.C. performed FISH, microscopy and tissue dissection.
One tonsil per child was prepared for immunofluorescence staining and three IF-positive samples underwent scanning electron microscopy and tissue Gram-staining.
Recent advances in both light microscopy and tissue culturing methods (Miyata et al. 2001; Noctor et al. 2001) allow direct time-lapse imaging of INM.
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A mean of 3.5 cores were obtained with 9.9 glomeruli per procedure for light microscopy (range, 0 to 32), 2.2 (range, 1 to 7) for electron microscopy, and adequate tissue for immunoflorescence available in 11 of 23 biopsies.
To corroborate the mRNA results, and to determine the origin and number of CC-chemokine-producing cells in SHIVSF162P4-infected maconfocalconfocal microscopy and MLN tissues were used.
Immunohistochemical staining of brain tissue regions rich in tau immunoreactivity was detected by light microscopy, and the same tissue region within the adjacent serial section was assessed by fluorescence microscopy to compare and determine whether the immunoreactive tau lesion colocalised with THK523 binding, indicated by a fluorescent signal.
12 days later, the endpoint of the cytopathic effect was determined by microscopy, and the 50% tissue culture infectious dose (TCID50) was calculated.
These samples are processed for array tomography [ 34] or electron microscopy and the remaining tissue is snap frozen on dry ice for biochemistry.
Thus, following experimental sepsis induction we examined gut blood flow by intravital microscopy and the gut tissue perfusion by laser Doppler, in order to detect the onset of the intestinal microcirculation changes at the acute phase of sepsis.
Related(20)
light and tissue
scan and tissue
microscopy and sheet
harvest and tissue
microscopy and cell
microscopy and texture
microscopy and imaging
microscopy and image
microscopy and sensing
microscopy and immunofluorescence
microscopy and microscope
microscopy and profilometry
microscopy and deconvolution
microscopy and laser
microscopy and culture
microscopy and energy
microscopy and immunohistochemistry
microscopy and flow
microscopy and X-ray
microscopy and embryo
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